In press

Papers accepted for publication

1. Remember: Ambroise Pare (1510-1590) - message for young surgeons

Cristian Constantin Popa, Andrei Alexandru Marinescu, Aurel George Mohan, Mircea Vicentiu Saceleanu, Alexandru Vlad Ciurea

Ambroise Pare was a renowned French barber-surgeon, considered by many to be the father of surgery. Originating from a family of barber-surgeons, he unraveled the secrets surgery at an early age, and he masters it by participating in the many military actions of the French Army of those times. As recognition of his merits, the kings of France proclaimed him their chief barber-surgeon and even president of the Royal College of French Surgeons. He was a doctor endowed with many qualities and contributed to the development of many medical specialties. Ambroise Pare has brought many contributions and innovations to the development of medicine. Worldwide, his name is closely related to the use of surgical hemostasis. As a true Professor, he offered his entire medical experience through the publication of 25 manuscripts that address various medical specialties. Perseverant and perfectionist by nature, Ambroise Pare represents a true role model for generations of surgeons to come.

Corresponding author: Mircea Vicentiu Saceleanu, MD, PhD; e-mail: vicentiu.saceleanu@gmail.com

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2. Microglial morphology determined with confocal and two-photon laser scanning microscopy

Smaranda Ioana Mitran, Emilia Burada, Cornelia-Andreea Tanasie, Nicolae Catalin Manea, Mihai Calin Ciorbagiu, Cecil Sorin Mirea, Tudor-Adrian Balseanu

Microglia are the first and main form of active immune defense in the nervous system. The immune status of microglia is directly correlated to their morphology. Therefore, microglia morphology is used to distinguish between active and surveilling microglia. For the actual paper, we used confocal laser scanning microscopy (cLSM) and two-photon laser scanning microscopy (2P-LSM), to investigate microglia morphology of 14-16 weeks old male, transgenic mice (n=6). After obtaining, in vivo and fixed tissue, single cells images, we manually tracked individually branch segments of normal microglia. The total number of branches and their overall length were analyzed. Additionally, the number and mean length of each branch order were measured. The overall microglia branching morphology was not different between the two acquisition methods. However, a higher number of fifth branches was observed using cLSM and 2P-LSM, in both fixed and in vivo tissue. Although results from the two methods are mainly comparable, small differences between them should be taken in consideration when formulating an activating/surveilling conclusion that is purely based on pure microscopic findings. Furthermore, in our opinion, due to their highly dynamic nature, microglia should be carefully labeled as resting or active, taking also into consideration the imaging method used to obtain the data.

Corresponding author: Tudor-Adrian Balseanu, Associate Professor, MD, PhD; e-mail: adrian.balseanu@umfcv.ro; Cecil Sorin Mirea, Lecturer, MD, PhD; e-mail: mirea_cecil@yahoo.com

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